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Evaluation of periodontal disease related bacteria from the implant-abutment interface

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Á¤´Ù¿î ( Jung Da-Un ) - ¼­¿ïƯº°½Ã º¸¶ó¸Åº´¿ø Ä¡°ú º¸Ã¶°ú
½ÉÇý¿µ ( Sim Hye-Young ) - ¼­¿ïƯº°½Ã º¸¶ó¸Åº´¿ø Ä¡°ú ±³Á¤°ú

Abstract


Purpose: The purpose of this study was to investigate the detection rate of putative periodontopathogens, Porphyromonas. gingivalitis, Prevotella. intermedius, Tannerella. forsythensis, Treponema. denticola, Actinobacillus. actionmycetemcomitans, related to implant-abutment interface microleakage.

Materials and Methods: Samples were taken from 27 subjects with sterilized paper points and were transported in 1*PBS. The detection of periodontopathogens were performed by polymerase chain reaction with species-specific primers based on 16S rDNA. The PCR products were cloned into pGEM-T easy vector and its nucleotides were sequenced in order to confirm the specificity.

Results: Our data showed that the detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in implant fixture was 59% and 82% in patients respectively. Detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in implant crevice was 44% and 82% in patients. Detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in tongue was 82% and 82% in patients.

Conclusion: Current implant systems cannot safely prevent microbial leakage and bacterial colonization of the inner part of the implant.

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Implant-abutment interface; Periodontopathogens; Microbial leakage; Porphyromonas gingivalisvalis

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